tre3g egfp vector Search Results


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Addgene inc tre3g promoter
Figure 2. Spatiotemporal characterization of the GFP-GluN2A transgene expression in GFP-GluN2A/tTA mice and its regulation using Dox. A, GFP-GluN2A transgene used to generate an inducible transgenic line expressing GFP-GluN2A from a <t>TRE3G</t> promoter. B–F, Representative coronal images depicting GFP-GluN2A transgene expression (Figure legend continues.)
Tre3g Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Spatiotemporal characterization of the GFP-GluN2A transgene expression in GFP-GluN2A/tTA mice and its regulation using Dox. A, GFP-GluN2A transgene used to generate an inducible transgenic line expressing GFP-GluN2A from a <t>TRE3G</t> promoter. B–F, Representative coronal images depicting GFP-GluN2A transgene expression (Figure legend continues.)
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Figure 2. Spatiotemporal characterization of the GFP-GluN2A transgene expression in GFP-GluN2A/tTA mice and its regulation using Dox. A, GFP-GluN2A transgene used to generate an inducible transgenic line expressing GFP-GluN2A from a <t>TRE3G</t> promoter. B–F, Representative coronal images depicting GFP-GluN2A transgene expression (Figure legend continues.)
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New England Biolabs nebuilder hifi dna assembly master mix
Figure 2. Spatiotemporal characterization of the GFP-GluN2A transgene expression in GFP-GluN2A/tTA mice and its regulation using Dox. A, GFP-GluN2A transgene used to generate an inducible transgenic line expressing GFP-GluN2A from a <t>TRE3G</t> promoter. B–F, Representative coronal images depicting GFP-GluN2A transgene expression (Figure legend continues.)
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New England Biolabs q5 high-fidelity dna polymerase
Figure 2. Spatiotemporal characterization of the GFP-GluN2A transgene expression in GFP-GluN2A/tTA mice and its regulation using Dox. A, GFP-GluN2A transgene used to generate an inducible transgenic line expressing GFP-GluN2A from a <t>TRE3G</t> promoter. B–F, Representative coronal images depicting GFP-GluN2A transgene expression (Figure legend continues.)
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Figure 2. Spatiotemporal characterization of the GFP-GluN2A transgene expression in GFP-GluN2A/tTA mice and its regulation using Dox. A, GFP-GluN2A transgene used to generate an inducible transgenic line expressing GFP-GluN2A from a TRE3G promoter. B–F, Representative coronal images depicting GFP-GluN2A transgene expression (Figure legend continues.)

Journal: The Journal of Neuroscience

Article Title: Increasing the GluN2A/GluN2B Ratio in Neurons of the Mouse Basal and Lateral Amygdala Inhibits the Modification of an Existing Fear Memory Trace

doi: 10.1523/jneurosci.1743-16.2016

Figure Lengend Snippet: Figure 2. Spatiotemporal characterization of the GFP-GluN2A transgene expression in GFP-GluN2A/tTA mice and its regulation using Dox. A, GFP-GluN2A transgene used to generate an inducible transgenic line expressing GFP-GluN2A from a TRE3G promoter. B–F, Representative coronal images depicting GFP-GluN2A transgene expression (Figure legend continues.)

Article Snippet: The TRE3G promoter was amplified from Addgene vector #27569 using the following DNA primers: FP: 5 -ATCCACGCGTTTTACTCCCTATCAGTGATAGAGAACG-3 RP: 5 -TAAAATCGATTTTACGAGGGTAGGA AGTGGTAC-3 and cloned into pTet-Splice ( MluI)-Dest-GFP-GluN2A via Mlu1 and Cla1 sites to generate pTRE3G-Splice ( MluI)-Dest-GFP-GluN2A.

Techniques: Expressing, Transgenic Assay